Pressure Induced Shifts in Spectral Properties of Pigment-Protein Complexes and Photosynthetic Organisms
نویسندگان
چکیده
Application o f elevated pressure (up to 1200 bars) results in a bathochrom ic shift o f the absorption bands o f photosynthetic pigments. The photosynthetic systems studied include: photosystem I particles, chloroplasts, acetone extracts o f chloroplasts, A U T particles and light harvesting particles o f Rh. sphaeroides, light harvesting particles o f R26. Sim ilar spectral changes are observed in all systems. The spectral shifts appear to be associated with pressure-induced changes in the local electric fields o f the pigments, rather than changes in the index o f refraction of the solvent.
منابع مشابه
Probing protein energy landscapes by optical spectroscopy in pigment-protein complexes involved in photosynthesis (September 28, 2015)
Probing protein energy landscapes by optical spectroscopy in pigment-protein complexes involved in photosynthesis In the past several years our group has been exploring protein energy landscapes in pigment-protein complexes involved in photosynthesis. These complexes offer a unique opportunity to explore native protein environments using optical spectroscopy methods, as chromophores are built i...
متن کاملLight Absorption and Energy Transfer in the Antenna Complexes of Photosynthetic Organisms.
The process of photosynthesis is initiated by the capture of sunlight by a network of light-absorbing molecules (chromophores), which are also responsible for the subsequent funneling of the excitation energy to the reaction centers. Through evolution, genetic drift, and speciation, photosynthetic organisms have discovered many solutions for light harvesting. In this review, we describe the und...
متن کاملIdentification of a single peridinin sensing Chl-a excitation in reconstituted PCP by crystallography and spectroscopy.
The peridinin-chlorophyll a-protein (PCP) of dinoflagellates is unique among the large variety of natural photosynthetic light-harvesting systems. In contrast to other chlorophyll protein complexes, the soluble PCP is located in the thylakoid lumen, and the carotenoid pigments outnumber the chlorophylls. The structure of the PCP complex consists of two symmetric domains, each with a central chl...
متن کاملMembrane protein stability: high pressure effects on the structure and chromophore-binding properties of the light-harvesting complex LH2.
Using the bacteriochlorophyll a (Bchl) cofactors as intrinsic probes to monitor changes in membrane protein structure, we investigate the response to high-pressure of the LH2 complexes from the photosynthetic bacteria Rhodobacter sphaeroides 2.4.1 and Rhodopseudomonas acidophila 10050. By FT-Raman spectroscopy, we demonstrate that high pressure does not induce significant distortion of the prot...
متن کاملDesign principles of photosynthetic light-harvesting.
Photosynthetic organisms are capable of harvesting solar energy with near unity quantum efficiency. Even more impressively, this efficiency can be regulated in response to the demands of photosynthetic reactions and the fluctuating light-levels of natural environments. We discuss the distinctive design principles through which photosynthetic light-harvesting functions. These emergent properties...
متن کامل